Method: reflectometric with test strips and reagent 0.25 - 2.50 g/l Reflectoquant®
The Reflectoquant Sucrose test is suitable for determination of sucrose in beverages, e. g. fruit/vegetable juice and refreshment drinks after appropriate sample pretreatment or dilution. Sucrose (saccharose) is cleaved by sucrose phosphorylase into fructose and glucose-1-phosphate. The latter is converted by phosphoglucomutase into glucose-6-phosphate. This is in turn oxidized by NAD under the catalytic effect of glucose-6-phosphate dehydrogenase to gluconate-6-phosphate. In the presence of diaphorase the NADH formed in the process reduces a tetrazolium salt to a blue formazan that is determined reflectometrically on the RQflex® 20. For the determination of only glucose please refer to our MQuant or Reflectoquant Glucose tests.
Linkage
Replaces: 1161410007; 1.16141.0007
Analysis Note
function control: entspricht Assay - standard 1: 0.26 - 0.34 mg/l - standard 2: 1.12 - 1.38 mg/l - standard 3: 1.80 - 2.20 mg/l
Legal Information
REFLECTOQUANT is a registered trademark of Merck KGaA, Darmstadt, Germany
RQFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10-13 - German Storage Class 10 to 13
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Reflectometric determination of sucrose in soft drinks using Reflectoquant® test strips and reflectometer for in-process control.
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