1.04190

Fuchsin acid (C.I. 42685)

• Empirical Formula (Hill Notation): C₂₀H₁₇N₃Na₂O₉S₃
• CAS Number: 3244-88-0
• Molecular Weight: 585.54
• UNSPSC Code: 12352106
• NACRES: NA.21

Properties

• form: solid
• Quality Level: 200
• composition: Dye, ≥60% spectrophotometric assay
• pH: 3-4 (20 °C, 10 g/L in H₂O)
• mp: >130 °C (Decomposition)
• solubility: 10 g/L
• bulk density: 920 kg/m³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,23H,21-22H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2*+1/p-2
• InChI key: WZRZTHMJPHPAMU-UHFFFAOYSA-L

Description

Application

Fuchsin acid (C.I. 42685) for microscopy Certistain^®, is a triarylmethane dye used, among others, in trichrome connective tissue stainings acc. to Mallory and van Gieson. Mallory developed a staining procedure for visualization of collagenous connective tissue, which has been modified and improved over the years.It enables a good differentiation of individual tissue components, provided that the tissue has been fixed in Zenker’s solution. As CERTISTAIN^® dye it is chemically quality-certified according to highest standards and strict specifications.

for microscopy Certistain^®

Analysis Note

Dye content (spectrophotometrically): ≥ 60 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (Hydrochloric acid 0.0025 mol/l): 540 - 549 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; hydrochloric acid 0.0025 mol/l): 780 - 1310
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable