05-1144

Anti-Talin Antibody, CT, clone TD77

clone TD77, from mouse

• Synonym(s): talin 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: TD77, monoclonal
• species reactivity: chicken, human, mouse
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1
• UniProt accession no.: Q9Y490
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: chicken ... Tln1(395194) ; human ... TLN1(7094) ; mouse ... Tln1(21894)

Description

General description

Talin consisting of a 47-kDa N-terminal head domain (residues 1-433) and a 190-kDa
C-terminal rod domain (residues 434-2541), links integrins to the actin cytoskeleton.

Specificity

Expected cross-reactivity in mouse and chicken.

Recognizes Human Talin, and cross reacts with Mouse and chicken Talin. The epitope recognized by this antibody is located within the C-terminal region of the protein (residues 2269-2541), which also contains an F-actin binding site homologous to that in the yeast actin-binding protein SIa2p.

Application

Detect Talin using this Anti-Talin Antibody, C-terminus, clone TD77 validated for use in WB, IH(P).

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Talin in human GI track tissue.

Quality

Evaluated by Western Blot in Jurkat cell lysates.
Western Blot Analysis: A 1:500 dilution of this lot detected Talin in 10 ug of Jurkat lysates.

Target description

270 kDa

Linkage

Replaces: MAB3264

Physical form

Format: Purified

Purified from Protein A Sepharose^™ chromatography

Purified immunoglobulin from Protein A Sepharose^™ chromatography. Liquid in 0.02M PBS pH 7.6, 0.25 M NaCl containing 0.1% sodium azide.

Storage and Stability

Maintain between 2 and 8°C for 1 year after date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable