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292648

Sigma-Aldrich

Dihydrofluorescein diacetate

97%

Synonym(s):

Diacetyldihydrofluorescein

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About This Item

Empirical Formula (Hill Notation):
C24H18O7
CAS Number:
35340-49-9
Molecular Weight:
418.40
Beilstein:
364167
MDL number:
MFCD00005056
UNSPSC Code:
12171500
PubChem Substance ID:
24857603
NACRES:
NA.47

Assay

97%

mp

213-215 °C (lit.)

solubility

acetone: 50 mg/mL

fluorescence

λex 272 nm in methanol
λex 490; λem 514 (Reaction product)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CC(=O)Oc1ccc2C(c3ccc(OC(C)=O)cc3Oc2c1)c4ccccc4C(O)=O

InChI

1S/C24H18O7/c1-13(25)29-15-7-9-19-21(11-15)31-22-12-16(30-14(2)26)8-10-20(22)23(19)17-5-3-4-6-18(17)24(27)28/h3-12,23H,1-2H3,(H,27,28)

InChI key

YKSJJXGQHSESKB-UHFFFAOYSA-N

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General description

Dihydrofluorescein diacetate (H2FDA) is an esterified derivative of dihydrofluorescein. H2FDA is one of the widely used fluorescent probes employed in the intracellular reactive oxygen species (ROS) detection.[1]

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKBF9112V
MKBF9112
13519BH
11402TF
08008KQ

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Chandan K. Sen, Lester Packer
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BMC plant biology, 13, 133-133 (2013-09-17)
In a previous study we have shown that wounding of Arabidopsis thaliana leaves induces a strong and transient immunity to Botrytis cinerea, the causal agent of grey mould. Reactive oxygen species (ROS) are formed within minutes after wounding and are
Ekrem Sulukan et al.
Chemosphere, 180, 77-85 (2017-04-10)
In this study, it has been investigated that the effects of glyphosate, which is a herbicide within organophosphate and unselective widely used in agriculture on enzyme activity of carbonic anhydrase, production of reactive oxygen species, cell apoptosis and body morphology
Emna Beneloujaephajri et al.
BMC plant biology, 13, 160-160 (2013-10-19)
Wounded leaves of Arabidopsis thaliana produce reactive oxygen species (ROS) within minutes after wounding and become resistant to the pathogenic fungus Botrytis cinerea at a local level. This fast response of the plants to the wound is called wound-induced resistance
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