Lumican: a new inhibitor of matrix metalloproteinase-14 activity.

Lumican: a new inhibitor of matrix metalloproteinase-14 activity.

FEBS letters (2014-10-12)

Katarzyna Pietraszek, Aurore Chatron-Colliet, Stéphane Brézillon, Corinne Perreau, Anna Jakubiak-Augustyn, Hubert Krotkiewski, François-Xavier Maquart, Yanusz Wegrowski

PMID25304424

ABSTRACT

We previously showed that lumican regulates MMP-14 expression. The aim of this study was to compare the effect of lumican and decorin on MMP-14 activity. In contrast to decorin, the glycosylated form of lumican was able to significantly decrease MMP-14 activity in B16F1 melanoma cells. Our results suggest that a direct interaction occurs between lumican and MMP-14. Lumican behaves as a competitive inhibitor which leads to a complete blocking of the activity of MMP-14. It binds to the catalytic domain of MMP-14 with moderate affinity (KD∼275 nM). Lumican may protect collagen against MMP-14 proteolysis, thus influencing cell-matrix interaction in tumor progression.

MATERIALS

Product Number

Brand

Product Description

PHR1321

Supelco

Sodium chloride, Pharmaceutical Secondary Standard; Certified Reference Material

PHR1428

Supelco

HEPES, Pharmaceutical Secondary Standard; Certified Reference Material

RDD002

Sigma-Aldrich

HEPES, anhydrous, free-flowing, Redi-Dri^™, ≥99.5%

RES6003H-B7

SAFC

HEPES

PHG0001

SAFC

HEPES

S5150

Sigma-Aldrich

Sodium chloride solution, 5 M in H₂O, BioReagent, for molecular biology, suitable for cell culture

S8032

Sigma-Aldrich

Sodium azide, BioXtra

H3375

Sigma-Aldrich

HEPES, ≥99.5% (titration)

H7523

Sigma-Aldrich

HEPES, BioXtra, pH 5.0-6.5 (1 M in H₂O), ≥99.5% (titration)

S5886

Sigma-Aldrich

Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

H6147

Sigma-Aldrich

HEPES, BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

H4034

Sigma-Aldrich

HEPES, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

S3014

Sigma-Aldrich

Sodium chloride, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

S6150

Sigma-Aldrich

Sodium chloride, tablet

S7653

Sigma-Aldrich

Sodium chloride, BioXtra, ≥99.5% (AT)

S6546

Sigma-Aldrich

Sodium chloride solution, 5 M

86826

Sigma-Aldrich

5-Carboxyfluorescein, BioReagent, suitable for fluorescence, ≥95% (HPLC)

71290

Sigma-Aldrich

Sodium azide, purum p.a., ≥99.0% (T)

21049

Supelco

Calcium standard for AAS, analytical standard, 1.000 g/L Ca⁺² in hydrochloric acid, traceable to BAM

71386

Sigma-Aldrich

Sodium chloride solution, BioUltra, for molecular biology, ~5 M in H₂O

71383

Sigma-Aldrich

Sodium chloride, tested according to Ph. Eur.

71387

Supelco

Sodium chloride, reference material for titrimetry, certified by BAM, >99.5%

54457

Sigma-Aldrich

HEPES, BioUltra, for molecular biology, ≥99.5% (T)

450006

Sigma-Aldrich

Sodium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

378860

Sigma-Aldrich

Sodium chloride, random crystals, optical grade, 99.9% trace metals basis

451940

Sigma-Aldrich

Sodium chloride-³⁵Cl, 99 atom % ³⁵Cl

204439

Sigma-Aldrich

Sodium chloride, 99.999% trace metals basis

S0817

Sigma-Aldrich

Sodium chloride solution, 0.85%

59222C

SAFC

Sodium chloride solution, 5 M

S6191

Sigma-Aldrich

Sodium chloride, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture