83264

HEPES solution

1 M in H₂O, liquid

• Synonym(s): HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• CAS Number: 7365-45-9
• MDL number: MFCD00006158
• UNSPSC Code: 12161700
• eCl@ss: 32129211
• PubChem Substance ID: 57652951
• NACRES: NA.25

Properties

• Product Name: HEPES buffer solution, 1 M in H₂O
• form: liquid
• Quality Level: 100
• concentration: 1 M in H₂O; 238 g/L HEPES in H₂O
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 5.5±0.5
• useful pH range: 6.8-8.2
• density: 1.07 g/mL at 20 °C
• application(s): diagnostic assay manufacturing
• SMILES string: OCCN1CCN(CCS(=O)(O)=O)CC1
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]

Application

HEPES has been used:

• As a component of SAPI medium used for the culturing of S. Typhymurium inoculum[3]
• To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI[4][5]
• As a component of dissection buffer for culturing rat cortical neurons[6]
• Along with MgCl₂, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses[7]
• As a component of Danieu′s aquarium water used in agarose gel preparation[8]

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves