ZRB2489

Anti-LTBP4 Antibody, clone 2M8 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): LTBP-4, Latent-transforming growth factor beta-binding protein 4
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: recombinant monoclonal
• description: clone 2M8 ; recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 173.44 kDa; observed mol wt ~200 kDa
• purified by: using Protein A
• species reactivity: mouse, human
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_001036009
• UniProt accession no.: Q8N2S1
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... LTBP4(8425)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2M8 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Latent-transforming growth factor β-binding protein 4 (LTBP4). It targets an epitope within 25 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal half of human Latent-transforming growth factor β-binding protein 4 (LTBP4).

Application

Quality Control Testing

Evaluated by Western Blotting in EL4 cell lysate.

Western Blotting Analysis: A 1:1.000 dilution of this antibody detected LTBP4 in EL4 cell lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected LTBP4 in Human kidney and Human epididymis tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LTBP4 in HepG2 and K562 cells.

Affinity Binding Assay: A representative lot of this antibody bound LTBP4 peptide with a KD of 8.6 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Latent-transforming growth factor beta-binding protein 4 (UniProt: Q8N2S1; also known as LTBP-4) is encoded by the LTBP4 gene (Gene ID: 8425) in human. LTBP4 is a key regulator of transforming growth factor β that controls TGF-β activation by maintaining it in a latent state during storage in extracellular space. It associates specifically via disulfide bonds with the latency-associated peptide (LAP), which is the regulatory chain of TGF-β. It is highly expressed in heart, skeletal muscle, pancreas, uterus, and small intestine. In human a long form (LTBP4-L) and a short isoform (LTBP4-S) have been described. LTBP4-L exhibits higher affinity for TGF-β1 compared to LTBP4-S form. Mice deficient in LTBP4-S form exhibit pulmonary emphysema, colorectal cancer, and cardiomyopathy. LTBP4 is reported to be upregulated in patients with chronic kidney disease and fibrotic mice kidneys. Down-regulation of LTBP4 expression is identified in melanoma tissues and cells and is associated with poor prognosis. Inhibition of LTBP4 is shown to reduce apoptosis and promote cell proliferation and metastasis. Four isoforms of LTBP4 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonal. (Ref.: Wang, L., et al. (2022). Front. Cell Dev. Biol. 9; 673904; Su, C-T., et al. (2021). Cell Death Dis. 12; Article 943; Lamar, KM., et al. (2016). PLOS Genetics 12(5); e1006019).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable