ZRB2180
Anti-Maxi K/BK channel Antibody, clone 3D15 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Calcium-activated potassium channel subunit alpha-1;BK channel;BKCA alpha;Calcium-activated potassium channel subfamily M subunit alpha-1;K(VCA)alpha;KCa1.1;Maxi K channel;MaxiK;Slo-alpha;Slo1;Slowpoke homolog;Slo homolog;hSlo
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
3D15, recombinant monoclonal
description
3D15 Clone
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 137.56 kDa
observed mol wt ~140 kDa
purified by
using Protein A
species reactivity
rat, human, mouse
species reactivity (predicted by homology)
sheep, horse, bovine, feline, monkey
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
C-terminal half
Protein ID accession no.
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... KCNMA1(3778)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 3D15 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Calcium-activated potassium channel subunit alpha-1 (Maxi K/BK channel). It targets an epitope within 19 amino acids from the C-terminal half.
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human Calcium-activated potassium channel subunit alpha-1 (Maxi K/BK channel).
Application
Quality Control Testing
Evaluated by Western Blotting in Mouse brain tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Maxi K/BK channel in Mouse brain tissue lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Maxi K/BK channel in Human brain and Rat brain tissue lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Maxi K/BK channel in human cerebellum tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Maxi K/BK channel peptide with a KD of 1.2 x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Maxi K/BK channel in A549 and C6 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Mouse brain tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Maxi K/BK channel in Mouse brain tissue lysate.
Evaluated by Western Blotting in Mouse brain tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Maxi K/BK channel in Mouse brain tissue lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Maxi K/BK channel in Human brain and Rat brain tissue lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Maxi K/BK channel in human cerebellum tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Maxi K/BK channel peptide with a KD of 1.2 x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Maxi K/BK channel in A549 and C6 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Mouse brain tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Maxi K/BK channel in Mouse brain tissue lysate.
Target description
Calcium-activated potassium channel subunit alpha-1 (UniProt: Q12791; also known as BK channel, BKCA alpha, Calcium-activated potassium channel subfamily M subunit alpha-1, K(VCA)alpha, KCa1.1, Maxi K channel, MaxiK, Slo-alpha, Slo1, Slowpoke homolog, Slo homolog, hSlo) is encoded by the KCNMA1 (also known as KCNMA, SLO) gene (Gene ID: 3778) in human. Maxi K is a homotetrameric, multi-pass membrane protein that contains five extracellular domains, seven transmembrane domains (S0-S6), four cytoplasmic domains, and one pore forming region (aa 336-358). With the exception of monocytes, it is widely expressed. It serves as the large-conductance Ca2+- and voltage-activated K+ channel that plays a role in cell signalling by interacting with a multiplicity of proteins at plasma membrane and intracellular organelles including the endoplasmic reticulum, nucleus, and mitochondria. Its structure consists of four -subunits, each formed by 7 transmembrane segments and a large C-terminus that constitutes about two thirds of the protein. The S4 segment (aa 265-285) that contains a series of positively charged amino acids at every third position serves as the voltage-sensor. Maxi K channel plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca2+, caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Maxi K is reported to be highly sensitive to iberiotoxin and charybdotoxin. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Toro, L., et al. (2014). Pflugers Arch. 466(5); 875-886).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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