ZRB1893

Anti-RPS25 Antibody, clone 3G15 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): 40S ribosomal protein S25 (UniProt: P62851; also known as Small ribosomal subunit protein eS25
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3G15, recombinant monoclonal
• description: 3G15 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 13.74 kDa; observed mol wt ~17 kDa
• purified by: using Protein A
• species reactivity: rat, human, mouse
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Unknown
• Protein ID accession no.: NP_001019
• UniProt accession no.: P62851
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... RPS25(6230)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3G15 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects 40S ribosomal protein S25 (RPS25).

Immunogen

His-tagged, full-length recombinant human 40S ribosomal protein S25 (RPS25).

Application

Quality Control Testing

Evaluated by Western Blotting in Rat Liver tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RPS25 in Rat Liver tissue lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RPS25 in A549 and NIH3T3 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RPS25 in human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound recombinant human RPS25 protein with a KD of 4.7 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RPS25 in HT-29 and U2OS cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in Rat Liver tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RPS25 in Rat Liver tissue lysate.

Target description

40S ribosomal protein S25 (UniProt: P62851; also known as Small ribosomal subunit protein eS25) is encoded by the RPS25 gene (Gene ID: 6230) in human. RPS25 plays a key role in several forms of unconventional translation including internal ribosome entry site (IRES)-mediated translation and ribosomal shunting. RPS25 is reported to interact with MDM2 and inhibit its E3 ligase activity that results in the reduction of MDM2-mediated p53 ubiquitination leading to p53 stabilization and activation. RPS25, MDM2, and p53 form a ternary complex following ribosomal stress. It is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats. However, reduction in RPS25 does not alter endogenous C9orf72 protein expression. Reduction in RPS25 levels by siRNA results in reduced levels of poly(GP) without affecting the number of RNA foci or levels of the different C9orf72 alternative transcript variants, including transcripts harboring the GGGGCC repeat. RPS25 also regulates translation initiation of hepatitis C virus and picornaviral IRES RNAs, downstream of 40S subunit recruitment. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Yamada, SB., et al. (2019). Nat. Neurosci. 11; 1383-1388; Zhnag, X., et al. (2013). Oncogene. 32; 2782-2791).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable