ZRB1401

Anti-Neuropeptide Y Antibody, clone 2B5 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): NPY
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2B5, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 10.85 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): mouse, rat
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable
• isotype: IgG
• epitope sequence: Unknown
• Protein ID accession no.: NP_000896
• UniProt accession no.: P01303
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... NPY(4852)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2B5 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Neuropeptide Y.

Immunogen

KLH-conjugated linear peptide corresponding to the mature Neuropeptide Y.

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human cerebral cortex tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Neuropeptide Y in human cerebral cortex tissue sections.

Tested applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Neuropeptide Y in SH-SY5Y cells.

Affinity Binding Assay: A representative lot of this antibody bound Neuropeptide Y with a KD of 5.8 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Immunohistochemistry (Paraffin) in human cerebral cortex tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Neuropeptide Y in human cerebral cortex tissue sections.

Target description

Pro-neuropeptide Y (UniProt: P01303) is encoded by the NPY gene (Gene ID: 4852) in human. Neuropeptide Y is one of the most abundant peptides in the nervous system and is also found in some chromaffin cells of the adrenal medulla. In the brain it is present in the cortex, hippocampus, hindbrain and hypothalamus. It is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. It is shown to be one the most potent orexigenic peptides that stimulates food intake with a preferential effect on carbohydrate intake. It decreases latency to eat, increases motivation to eat and delays satiety by augmenting meal size. Its effects on feeding are mediated through the Y1 and Y5 receptors. Neuropeptide Y also acts as a vasoconstricting peptide that is released from sympathetic nerves in the heart and affects coronary artery constriction and myocardial contraction. It is also reported to potentiate the vasoconstrictor effects of other extracellular messengers, including a-adrenergic agonists and angiotensin II. It is also linked to inhibition of acetylcholine release from parasympathetic nerve endings in the heart. Neuropeptide Y is synthesized as a pre-propeptide with a signal peptide (aa 1-28) and a C-flanking peptide (aa 68-97) that are subsequently cleaved off to produce the active peptide (aa 29-64). This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tan, CMJ., et al. (2018). Front. Physiol. 9; 1281; Beck, B. (2006). Philos. Trans. R. Soc. Lond. B Biol. Sci. 361(1471); 1159-1185).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable