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Z701270

Sigma-Aldrich

PCR Primer: A Laboratory Manual, 2nd ed.

each technique is presented with extensive background information, advice, and troubleshooting

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About This Item

ISBN-10:
0-87969-654-0
ISBN-13:
978-0-87969-654-2
UNSPSC Code:
55101509

publication info

C. Dieffenbach and G. Dveksler, ed., Cold Spring Harbor Laboratory Press, 2003, 700 pp., soft cover

manufacturer/tradename

Cold Spring Harbor

technique(s)

PCR: suitable

General description

This second edition is a much praised and widely used manual that has been entirely revised and updated. Each technique is presented with extensive background information, advice, and troubleshooting.

All current applications of PCR are covered in protocols that have the hallmark reliability of the previous edition.

Table of Contents

Section 1. Introduction to PCR
Section 2. Sample Preparation
Section 3. RNA PCR Methods
Section 4. Detection of PCR Products: Specialized Applications
Section 5. Analysis of Differential Gene Expression by PCR
Section 6. Cloning by PCR
Section 7. Cloning of PCR Products
Section 8. Mutagenesis by PCR
Appendices

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