T5425

Transforming Growth Factor-β3 human

TGF-β3, recombinant, expressed in baculovirus infected Sf21 cells, powder, suitable for cell culture

• Synonym(s): hTGF-β3, TGF-β3
• MDL number: MFCD00213054
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in baculovirus infected Sf21 cells
• Assay: ≥97% (SDS-PAGE)
• form: powder
• potency: 0.01-0.09 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• mol wt: predicted mol wt ~25 kDa
• packaging: pkg of 2 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: <0.01 EU/μg
• UniProt accession no.: P10600
• storage temp.: −20°C
• Gene Information: human ... TGFB3(7043)

Description

General description

The TGFβ3 (transforming Growth Factor-β3) gene is mapped to human chromosome 14q24.3.

Application

Transforming Growth Factor-β3 human has been used to study the role of TGF-β3 in healing tendon-to-bone insertion and in chondrogenic differentiation.

Biochem/physiol Actions

TGF-β (transforming growth factor-β3) isoforms are significantly involved in inducing inflammatory responses. It is associated with vascular diseases, wound healing and ameliorating effect in venous leg ulcer. TGF-β3 has an antagonistic effect on TGF-β1 and reduces the degree of collagen type I deposition and fibroblast differentiation. As a result TGF-β3 might lead to ulcer chronicity due to hindered myofibroblasts formation. TGF-β is a key player in tumor formation and progression. Increased TGF-β3 expression is observed in cancer and adipose tissue fibrosis. TGF-β3 is crucial in tissue regeneration and scarless tissue repair.

TGF-β3 is less prevalent in natural expression than either TGF-β1 or TGF-β2, but it is the most abundant mRNA expressed in chick embryos. It is also expressed in human umbilical cord, in a variety of mesenchymal cells of human and rodent origin, and in several human carcinoma cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.

Analysis Note

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves