SRP4972

GHBP human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

• Synonym(s): GH receptor, GHBP, GHR, Somatotropin receptor
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: ~28.1 kDa
• packaging: pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: P10912
• UniProt accession no.: P10912
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... GHR(2690)

Description

General description

GHBP is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. A common alternate allele of this gene, called GHRd3, lacks exon three and has been well-characterized. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. Human Recombinant GHBP expressed from E. coli is a single, non-glycosylated, polypeptide chain containing 237 amino acids and having a molecular mass of 28.1 kDa. GHBP is purified by proprietary chromatographic techniques.
The gene encoding growth hormone receptor (GHR or GHBP) is localized on human chromosome 5p13.1-p12, with nine exons. It possesses a cytoplasmic and an extracellular domain for protein binding.

Physical form

GHBP was lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO3.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in sterile dH₂O not less than 100 μg/ml. This solution can then be diluted into other aqueous buffers.