SRP0153

SMYD3 (full length) Active human

recombinant, expressed in baculovirus infected insect cells, ≥50% (SDS-PAGE)

• Synonym(s): KMT3E, Lysine N-methyltransferase 3E, SET and MYND domain containing 3, ZMYND1, Zinc finger MYND domain-containing protein 1
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥50% (SDS-PAGE)
• form: aqueous solution
• mol wt: 50 kDa
• packaging: pkg of 20 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: >0.02 mg/mL
• NCBI accession no.: BC031010
• UniProt accession no.: Q9H7B4
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... SMYD3(64754)

Description

General description

Human SMYD3 (GenBank Accession No. BC031010), full length, with N-terminal DDDDK tag (FLAG), MW = 50 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 100 μg/ml flag peptide, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.