Granzyme B human recombinant, expressed in insect cells, Molecular weight is 32kDa as per Uniprot P10144. Please see the link below for more information:
https://www.uniprot.org/uniprot/P10144
SRE0021
Granzyme B human
recombinant, expressed in insect cells, ≥90% (SDS-PAGE)
Synonym(s):
Granzyme B human, Cytotoxic T-lymphocyte proteinase 2, Granzyme-2, GranzymeB, Lymphocyte protease, SECT, T-cell serine protease 1-3E
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5 μG
₪2,682.00
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5 μG
₪2,682.00
About This Item
UNSPSC Code:
12352204
NACRES:
NA.32
Recommended Products
biological source
human
Quality Level
recombinant
expressed in insect cells
Assay
≥90% (SDS-PAGE)
form
lyophilized powder
potency
>0.004 units/mg
specific activity
≥40 units/μg
packaging
pkg of 5 μg
UniProt accession no.
shipped in
wet ice
storage temp.
−70°C
Gene Information
human ... GZMB(3002)
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General description
Granzyme B (GzmB) is a serine protease which is stored in granules of activated cytotoxic T cells and NK cells. GZMB codes for granzyme B.[1]
Biochem/physiol Actions
Granzyme B is considered as the marker of activated CTLs (cytotoxic T-cells), which mediates target-cell apoptosis.[1] It induces apoptosis in caspase-dependent and caspase-independent manner.[2] After contact with target cells, granzyme B is directionally exocytosed, and then enters the target cell with assistance from perforin. Granzyme B specifically cleaves after Asp residues, and particularly processes and activates various pro-caspases. This induces apoptosis in the target cell.
Unit Definition
One unit is defined as the amount of enzyme that hydrolyzes 1 pmol/min of IETD-AFC per min at 37°C in 50 mM HEPES, pH 7.5 containing 10 mM CaCl2.
Physical form
Lyophilized powder, containing HEPES buffer (original solution at pH 7.5)
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Granzyme B enters the mitochondria in a Sam50-, Tim22-and mtHsp70-dependent manner to induce apoptosis
Chiusolo V, et al.
Cell Death and Differentiation, 24(4), 747-747 (2017)
Risk of generalized vitiligo is associated with the common 55R-94A-247H variant haplotype of GZMB (encoding granzyme B).
Ferrara TM, et al.
The Journal of Investigative Dermatology, 133(6), 1677-1677 (2013)
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What is the molecular weight?
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To whom it may concern, we have recently purchased the granzyme B (SRE0021) and its substrate (SCP0158). Is the enzyme in its active form? What are the best condition for resuspension? Thank you.
1 answer-
The product is provided in active form. It does not require activation. Use 50 µl sterile deionized water to dissolve 5 µg protein to a final concentration of 0.1 µg protein/µl. This solution is stable for 1 year at –70 °C.
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