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SAB4200669

Sigma-Aldrich

Anti-Digoxin antibody, Mouse monoclonal

clone DI-22, purified from hybridoma cell culture

Synonym(s):

12β-Hydroxydigitoxin, Monoclonal Anti-Digoxin antibody produced in mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

DI-22, monoclonal

species reactivity

human, mouse, rat

concentration

1.0 mg/mL
~1 mg/mL

technique(s)

ELISA: 0.15-0.3 μg/mL

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma DI-22 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with digoxin conjugated to keyhole limpet hemocyanin (KLH).

Immunogen

immunized with Digoxin

Application

Monoclonal Anti-Digoxin antibody produced in mouse has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • flow cytometry
  • immunofluorescence
  • DNA hybridization
  • in-situ hybridization (ISH)

Biochem/physiol Actions

Digoxin is an uncharged glycoside which is given to humans with congestive heart failure. It enhances the force of myocardial contraction. Anti-digoxin antibodies can be used to reverse the effects of digoxin.
Unlike other oral inotrope, digoxin does not promote mortality. It inhibits sodium potassium ATPase membrane pump.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Digoxin
Eichhorn EJ and Gheorghiade M
Progress in Cardiovascular Diseases, 44(4), 251-266 (2002)
High-affinity monoclonal antibodies to the cardiac glycoside, digoxin.
M M Hunter et al.
Journal of immunology (Baltimore, Md. : 1950), 129(3), 1165-1172 (1982-09-01)

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