R9782

RS-1

≥98% (HPLC), RAD51 stimulator, powder

• Synonym(s): 3-[(benzylamino)sulfonyl]-4-bromo-N-(4-bromophenyl)benzamide, 4-Bromo-N-(4-bromophenyl)-3-[[(phenylmethyl)amino]sulfonyl]-benzamide, RAD51-stimulatory compound 1
• Empirical Formula (Hill Notation): C₂₀H₁₆Br₂N₂O₃S
• CAS Number: 312756-74-4
• Molecular Weight: 524.23
• UNSPSC Code: 12352200
• PubChem Substance ID: 329824048
• NACRES: NA.77
• MDL number: MFCD00348720
• Assay: ≥98% (HPLC)
• Form: powder

Properties

• Product Name: RS-1, ≥98% (HPLC)
• assay: ≥98% (HPLC)
• form: powder
• color: off-white to light tan
• solubility: DMSO: ≥10 mg/mL
• storage temp.: room temp
• SMILES string: Brc1ccc(NC(=O)c2ccc(Br)c(c2)S(=O)(=O)NCc3ccccc3)cc1
• InChI: 1S/C20H16Br2N2O3S/c21-16-7-9-17(10-8-16)24-20(25)15-6-11-18(22)19(12-15)28(26,27)23-13-14-4-2-1-3-5-14/h1-12,23H,13H2,(H,24,25)
• InChI key: SWKAVEUTKGKHSR-UHFFFAOYSA-N

Description

Application

RS-1 has been shown to enhance CRISPR genome editing efficiency. To see other small molecule CRISPR enhancers, visit sigma.com/CRISPR-enhancers.

RS-1 has been used:

• as a homology-directed repair (HDR) agonist to study its effects on in human hematopoietic stem/progenitor cells (HSPCs)[1]
• as HDR agonist to analyze its effects on DNA repair modulation in human induced pluripotent stem (iPS) cells[2]
• as RAD51 agonist to study its effects on double-stranded break repair[3]

Biochem/physiol Actions

RS-1 (RAD51-stimulatory compound 1) is a stimulator of the human homologous recombination (HR) protein RAD51. RS-1 stimulates binding of hRAD51 to single stranded DNA (ssDNA) and enhances recombinogenic activity by stabilizing the active form of hRAD51 filaments without inhibiting hRAD51 ATPase activity. RS-1 has been shown to enhance CRISPR-Cas9 knock-in efficiency in HEK293A cells and has been shown to enhance both TALEN and Cas9-mediated knock-in efficiency in rabbits.

RS-1 is a stimulator of the human homologous recombination protein RAD51.

RS-1 is a sulfonamido-benzamide compound.[4]

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable