P8913
Phospholipase A2 from bovine pancreas
lyophilized powder, ≥20 units/mg protein
Synonym(s):
Lecithinase A, PLA2, Phosphatidylcholine 2-acylhydrolase
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About This Item
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form
lyophilized powder
specific activity
≥20 units/mg protein
composition
Protein, 5-20% biuret
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... PLA2G1B(282457)
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General description
Phospholipase A2 from bovine pancreas is a calcium-dependent lipolytic enzyme.
Application
Phospholipase A2 from bovine pancreas has been used to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation and to study the impact of phospholipase A2 on tubular cell triglyceride levels.
Biochem/physiol Actions
Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.
Packaging
Package size based on protein content
Unit Definition
One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 and 37 °C.
Physical form
Contains Tris buffer salts.
Analysis Note
Protein determined by biuret.
inhibitor
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Renal tubular triglyercide accumulation following endotoxic, toxic, and ischemic injury.
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Triglyceride accumulation in injured renal tubular cells: alterations in both synthetic and catabolic pathways.
Johnson AC
Kidney International, 67(6), 2196-2209 (2005)
Phospholipase A2 activity can protect renal tubules from oxygen deprivation injury.
Zager RA
Proceedings of the National Academy of Sciences of the USA, 90(17), 8297-8301 (1993)
J J Seilhamer et al.
The Journal of biological chemistry, 264(10), 5335-5338 (1989-04-05)
Synovial fluid from arthritic patients contains multiple forms of phospholipase A2 (PLA2), as resolved by high performance liquid chromatography (Seilhamer, J.J., Plant, S., Pruzanski, W., Schilling, J., Stefanski, E., Vadas, P., and Johnson, L. K. (1989) J. Biochem. (Tokyo), submitted
Intracellular phospholipases A.
H van den Bosch
Biochimica et biophysica acta, 604(2), 191-246 (1980-09-30)
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