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| Size/SKU | Availability | Price |
|---|---|---|
25 units | Please contact Customer Service for Availability | ₪965.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥3.0 units/mg solid
Biological source:
yeast
biological source
yeast
form
lyophilized powder
specific activity
≥3.0 units/mg solid
mol wt
52 kDa
storage temp.
2-8°C
General description
6-Phosphogluconic Dehydrogenase from yeast comprises of a NADP+ binding domain, α helical hydrophobic interaction domain and a C-terminal domain. It exists as dimer and the C-terminal region is crucial for the substrate binding and release.
Application
6-Phosphogluconic Dehydrogenase from yeast has been used in hexokinase (HK) and xylose reductase (XR) activities in cell lysate.
Biochem/physiol Actions
Enzyme activity is determined by measuring the reduction of NAD+ or NADP+. High levels of NADPH are believed to inhibit the enzyme, while 6-phosphogluconate acts to activate the enzyme[1].
Other Notes
One unit will oxidize 1.0 μmole of 6-phospho-D-gluconate to D-ribulose 5-phosphate and CO2 per min at pH 7.4 at 37 °C in the presence of NADP+.
1 of 1
This Item | |||
|---|---|---|---|
| biological source yeast | biological source bacterial (Leuconostoc mesenteroides) | biological source bakers yeast | biological source - |
| specific activity ≥3.0 units/mg solid | specific activity >= 550 units/mg protein (biuret) | specific activity ≥200 units/mg protein | specific activity 200-400 units/mg protein (modified Warburg-Christian) |
| form lyophilized powder | form lyophilized powder | form ammonium sulfate suspension | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| mol wt 52 kDa | mol wt 128 kDa | mol wt 128 kDa | mol wt 128 kDa |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 300 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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