P2732

Monoclonal Anti-p120^ctn (Catenin-related) antibody produced in mouse

clone 6H11, tissue culture supernatant

• Synonym(s): Anti-BCDS2, Anti-CAS, Anti-CTNND, Anti-P120CAS, Anti-P120CTN, Anti-p120, Anti-p120(CAS), Anti-p120(CTN)
• MDL number: MFCD02097107
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: tissue culture supernatant
• antibody product type: primary antibodies
• clone: 6H11, monoclonal
• contains: 15 mM sodium azide
• species reactivity: bovine, canine, chicken, mouse, rat, human
• technique(s): immunoprecipitation (IP): suitable; indirect ELISA: suitable; indirect immunofluorescence: 1:100 using cultured Madin Darby canine kidney (MDCK) epithelial cells; western blot: 1:10,000 using whole cell extract of cultured chicken fibroblasts
• isotype: IgG1
• UniProt accession no.: O60716
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CTNND1(1500) ; mouse ... Ctnnd1(12388) ; rat ... Ctnnd1(311163)

Description

General description

Monoclonal Anti-p120ctn (Catenin-related) (mouse IgG1 isotype) is derived from the 6H11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an A/J mouse immunized with a recombinant N-terminal fragment of murine p120ctn. Catenin δ-1 (p120ctn) contains a central armadillo repeat domain (ARM domain) and is structurally similar to β-catenin and plakoglobin. At least six alternatively spliced p120ctn isoforms are generated and differentially expressed in different cell types.

Specificity

Reacts specifically with the N-terminal region of p120^ctn and recognizes isoforms 1 and 2, but not isoforms 3 or 4. In immunoblotting, the antibody recognizes a 120 kDa band, and an additional very weak doublet at 200 kDa.

Immunogen

recombinant N-terminal fragment of murine p120^ctn.

Application

Monoclonal Anti-p120ctn (Catenin-related) antibody produced in mouse has been used in:

• immunoblotting
• enzyme linked immunosorbent assay (ELISA),
• immunocytochemistry
• immunoprecipitation

Biochem/physiol Actions

The 120 kD catenin-related protein p120ctn (also known as catenin p120ctn, p120cas and pp120 src substrate), was originally described as a prominent substrate for the sarcoma (Src) oncoprotein and for a variety of receptor tyrosine kinases (RTKs) including those for epidermal growth factor (EGF), platelet derived growth factor (PDGF), and colony stimulating factor-1 (CSF1). Like β-catenin and plakoglobin, it binds directly to the cytoplasmic domain of epithelial (E)-cadherin via the ARM domain and co-precipitates in multiprotein complexes containing E-cadherin or other cadherins such as neural (N)- or placental (P)- cadherins, depending on the cell type. However, unlike β-catenin and plakoglobin, p120ctn does not appear to bind α-catenin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable