N6133

4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside

≥99% (TLC), powder

• Synonym(s): pNP-chitobiose
• Empirical Formula (Hill Notation): C₂₂H₃₁N₃O₁₃
• CAS Number: 7284-16-4
• Molecular Weight: 545.49
• Beilstein: 1445509
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897770
• NACRES: NA.32

Properties

• product name: 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside, ≥99% (TLC)
• Quality Level: 200
• Assay: ≥99% (TLC)
• form: powder
• solubility: DMF: 24.50-25.50 mg/mL
• storage temp.: 2-8°C
• SMILES string: CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]2[C@H](O)[C@@H](NC(C)=O)[C@@H](O[C@@H]2CO)Oc3ccc(cc3)[N+]([O-])=O
• InChI: 1S/C22H31N3O13/c1-9(28)23-15-18(31)17(30)13(7-26)36-22(15)38-20-14(8-27)37-21(16(19(20)32)24-10(2)29)35-12-5-3-11(4-6-12)25(33)34/h3-6,13-22,26-27,30-32H,7-8H2,1-2H3,(H,23,28)(H,24,29)/t13-,14-,15-,16-,17-,18-,19-,20-,21-,22+/m1/s1
• InChI key: HWBFEVWOQMUQIE-MVEDJEFUSA-N

Description

Application

4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside has been used to measure the exochitinase activity of DOI, a novel protein from Chinese yam (Dioscorea opposita Thunb.) and exochitinase activity of ChiEn3 from Coprinopsis cinerea. It has also been used to determine the activity of a chitinolytic enzyme, N-acetyl-β-D-glucosaminidases.

Biochem/physiol Actions

4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside is a substrate for determining exochitinase activity (chitobiosidase activity).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves