Skip to Content
Merck
All Photos(3)

Documents

MAK051

Sigma-Aldrich

Aconitase Activity Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Aconitase Enzyme Activity Kit

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

neurological disorders; cardiovascular diseases; cancer

storage temp.

2-8°C

Gene Information

human ... ACO1(48) , ACO2(50)
mouse ... ACO1(11428) , ACO2(11429)
rat ... ACO1(50655) , ACO2(79250)

General description

The aconitases are a family of iron-sulfur containing enzymes that catalyzes the isomerization of citrate to isocitrate. Two aconitases, encoded by different genes, have been identified in eukaryotes. Aconitase 1 (ACO1, c-aconitase) is a cytosolic enzyme while Aconitase 2 (ACO2, m-aconitase) is a mitochondrial enzyme that functions in the tricarboxylic acid cycle. Aconitases are reversibly inactivated by oxidative stress, and aconitase activity in cells and tissues has been used as a biomarker for oxidative damage.

Application

Aconitase Activity Assay Kit has been used to measure the activity of cytosolic and mitochondrial aconitase enzyme.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of aconitase activity in biological samples including tissue and cells

Principle

The Aconitase Activity Assay kit provides a simple and direct procedure for measuring Aconitase activity in a variety of samples. Aconitase activity is determined in a coupled enzyme reaction in which citrate is converted to isocitrate by aconitase. This results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of aconitase is the amount of enzyme that will isomerize 1.0 μmole of citrate from isocitrate per minute at pH 7.4 at 25 °C.

replaced by

Product No.
Description
Pricing

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H314,H334

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 1

1 of 1

(+)-Potassium Ds-threo-isocitrate monobasic ≥98.0% (NT)

Sigma-Aldrich

58790

(+)-Potassium Ds-threo-isocitrate monobasic

Jayasimman Rajendran et al.
EMBO molecular medicine, 11(1) (2018-12-12)
Alternative oxidase (AOX) is a non-mammalian enzyme that can bypass blockade of the complex III-IV segment of the respiratory chain (RC). We crossed a Ciona intestinalis AOX transgene into RC complex III (cIII)-deficient Bcs1l
Vincenzo Maione et al.
Biochimica et biophysica acta. General subjects, 1862(9), 1980-1987 (2018-05-31)
The CIA2A protein, in complex with CIAO1, has been proposed to be exclusively implicated in the maturation of cytosolic aconitase. However, how the CIA2A-CIAO1 complex generates active aconitase is still unknown and the available structural information has not provided any
Evaluation of the adaptogenic potential exerted by ginsenosides Rb1 and Rg1 against oxidative stress-mediated neurotoxicity in an in vitro neuronal model.
Carlos F M, et al.
PLoS ONE, 12(8), e0182933-e0182933 (2017)
NFS1 undergoes positive selection in lung tumours and protects cells from ferroptosis.
Alvarez S W, et al.
Nature, 551(7682), 639-639 (2017)
François Beaufay et al.
mBio, 11(4) (2020-07-30)
Maintaining cellular iron homeostasis is critical for organismal survival. Whereas iron depletion negatively affects the many metabolic pathways that depend on the activity of iron-containing enzymes, any excess of iron can cause the rapid formation of highly toxic reactive oxygen
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service