M7194

Monoclonal Anti-MEKK4 antibody produced in mouse

~2 mg/mL, clone MEKK4-338, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-MAP3K4
• MDL number: MFCD08705590
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: MEKK4-338, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~182 kDa
• species reactivity: human, rat, mouse
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 10-20 μg/mL using total cell extract of HEK293T cells
• isotype: IgG2b
• UniProt accession no.: Q9Y6R4
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAP3K4(4216) ; mouse ... Map3k4(26407) ; rat ... Map3k4(308106)

Description

General description

Monoclonal Anti-MEKK4 (mouse IgG2b isotype) is derived from the hybridoma MEKK4-338 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human MEKK4. MAP kinase 4 (MEKK4 also called MAP3K4, is the human homolog of the S. cerevisiae Ssk2 and Ssk22 MAPKKKs. The protein has 1607 amino acids with a protein kinase catalytic domain at the C-terminus that is 98, 42, 36 and 41 percent identical to the kinase domains of mouse MEKK4, Serine/threonine-protein kinase proteins (SSK22 - Ssk2, Ssk22) and S. pombe Wik1, respectively. A non-kinase domain that has a regulatory function is located at the N-terminal part of the protein. The protein is expressed mainly in heart, placenta, skeletal muscle and pancreas.

Specificity

Monoclonal Anti-MEKK4 antibody is specific for human, rat and mouse MEKK4 (approx. 182 kDa).

Immunogen

synthetic peptide corresponding to amino acids 1081-1095 of human MEKK4, conjugated to KLH.

Application

Monoclonal Anti-MEKK4 antibody produced in mouse has been used in:

• western blotting
• ELISA
• immunocytochemistry

Biochem/physiol Actions

MAP kinase 4 (MEKK4) -null embryos developed highly penetrant neural tube defects with exencephaly and spina bifida. During neural tube development, it regulates MAP kinase 4 (MKK4) activity and apoptotic cell death.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves