M6697

Anti-MLKL (58-70) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-mixed lineage kinase domain-like (Homo sapiens)
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 28

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~54 kDa
• species reactivity: human
• technique(s): western blot: 1:250-1:500
• UniProt accession no.: Q8NB16
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MLKL(197259)

Description

General description

MLKL (mixed lineage kinase domain-like) is a protein that belongs to the protein kinase superfamily. It facilitates necrosis signaling downstream of the kinase RIP3 (receptor-interacting serine-threonine kinase 3) . Anti-MLKL (58-70) antibody can be used in western blotting. Rabbit anti- MLKL (58-70) antibody reacts specifically with MLKL protein.

MLKL is encoded by the gene mapped to human chromosome 16q23. Activated MLKL is localized on the cell membrane.

Immunogen

synthetic peptide corresponding to amino acids 58-70 of human MLKL.

Application

Anti-MLKL (58-70) antibody produced in rabbit has been used in western blotting.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

western blot: 1:250-1:500

Biochem/physiol Actions

Mixed lineage kinase domain-like protein (MLKL) primarily causes receptor-interacting protein (RIP) kinase-dependent necroptosis. However, during hepatitis, it results in programmed hepatocellular necrosis, which is independent of RIPK3. MLKL also participates in endosomal trafficking and in the formation of extracellular vesicles.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)