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M4280

Sigma-Aldrich

Anti-Mouse IgG (Fc specific) antibody produced in goat

2.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti Mouse Fc Antibody, Anti Mouse Fc Antibody - Anti-Mouse IgG (Fc specific) antibody produced in goat

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

concentration

2.0 mg/mL

technique(s)

indirect ELISA: 1:20,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulins have two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Immunogen

Fc fragment of IgG purified from mouse.

Application

Anti-Mouse IgG (Fc specific) antibody produced in goat has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • flow cytometry
  • immunoprecipitation
  • immunoblotting

Biochem/physiol Actions

IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. The antigen binding activity is mediated by the variable Fab region of IgG antibodies, whereas the effector activities are stimulated by the Fc domain. Anti-mouse IgG (Fc specific) antibody can be used to detect the optimal capture agents.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. It mainly helps in immune defense. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with bovine, horse or human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lina Y Dimberg et al.
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Analytical biochemistry, 383(1), 52-60 (2008-09-11)
The production of antibodies for diagnostic and therapeutic applications is a major focus for biotechnology and pharmaceutical companies, and it requires the development of fast, high-throughput methodologies for screening and selecting appropriate candidate antibodies for development. These candidates must have
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Journal of inflammation (London, England), 12, 21-21 (2015-04-14)
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T Groot Kormelink et al.
Allergy, 67(9), 1165-1172 (2012-07-07)
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A genome-wide loss-of-function screen identifies SLC26A2 as a novel mediator of TRAIL resistance
Dimberg LY, et al.
Molecular Cancer Research, 15(4), 382-394 (2017)

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