I4401

Interferon-αA/D human

≥95%, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

• Synonym(s): IFN-αA/D
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD01635116

Properties

• biological source: human
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥95%
• form: buffered aqueous solution
• mol wt: 19 kDa
• packaging: pkg of 100K units
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• color: colorless
• UniProt accession no.: P01563
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... IFNA2(3440)

Description

Application

Interferon-αA/D human has been used:

• for identification of coronavirus replicase inhibitors[1]
• in combination with ACH-806 [1-(4-pentyloxy-3-trifluoromethylphenyl)-3-(pyridine-3-carbonyl)thiourea] to study the effect on hepatitis C virus (HCV) replication[2]
• in combination with vitamin D to check reduction in HCV protein production in cell culture[3]

Biochem/physiol Actions

Interferon-αA/D (Bg/II), also known as Universal Type I Interferon, is an α interferon hybrid that crosses the species barrier. The Bg/II denotes the restriction enzyme site from which this hybrid is constructed from recombinant human interferons αA and αD. Interferon-αA/D has been proven to be active on many mammalian cells. It can substitute for human, monkey, mouse, bovine, rat, cat, dog, rabbit, sheep, goat, horse, pig or hamster type I interferon (α, β, ω and τ). Type I Interferons are a closely related family of 165-172 amino acid proteins that are produced by leukocytes (α subtypes), fibroblasts (β subtypes), lymphocytes (ω subtypes), and ruminant embryos (τ subtypes). This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

Physical form

Solution in phosphate buffered saline containing 0.1% bovine serum albumin

Analysis Note

The biological activity is determined in the cytopathic effect inhibition assay using MDBK cells with vesicular stomatitis virus (VSV).[4][5] A cytopathic effect of 50% is produced with 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for human interferonαA.[6]

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves