H3291

Anti-HOXD4 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-HHO.C13, Anti-HOX-4B, Anti-HOX-5.1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~35 kDa
• species reactivity: mouse, human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 1.5-3 μg using lysates of HEK-293T cells over-expressing human HOXD4; indirect immunofluorescence: 0.25-0.5 μg/mL using paraformaldehyde-fixed HEK-293T cells over-expressing human HOXD4; western blot: 0.5-1 μg/mL using mouse brain extract
• UniProt accession no.: P09016
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... HOXD4(3233)

Description

General description

The Homeobox D4 (HOXD4) gene is localized in mesodermal tissues and the central nervous system (CNS). The HOXD4 gene is located on the human chromosome at 2q31.1.

Specificity

Anti-HOXD4 (N-terminal) recognizes human and mouse- HOXD4 (also known as HOX-4B, HOX-5).

Application

Anti-HOXD4 (N-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

The Homeobox D4 (HOXD4) gene participates in tumorigenesis of the central nervous system. This gene acts as a biomarker in the diagnosis of gliomas. HOXD4 is involved in the evolution of spine and dura mater. It also participates in the etiology and pathogenesis of spinal extradural arachnoid cyst (SEDAC).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable