GE28-9365-41
HiPrep™ DEAE Fast Flow 16/10
Cytiva 28-9365-41
About This Item
Recommended Products
ligand
diethylaminoethyl
description
Ion Exchanger Type (value)
packaging
pkg of 20 mL
manufacturer/tradename
Cytiva 28-9365-41
parameter
1.5 bar (22 psi) (Over the Packed Bed During Operation)
2 ml/min - 10 ml/min flow rate
22 psi
bed size
16 mm × 100 mm
bed volume
20 mL
column I.D.
16 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14
working range
2-12
suitability
suitable for bioprocess medium
General description
HiPrep™ DEAE FF 16/10 is a prepacked, ready to use column for ion exchange chromatography. It provide sfast, preparative separations of proteins and other biomolecules.
Features and Benefits
- 20 mL HiPrep™ columns provide fast, reliable, and reproducible preparative ion exchange separations.
- Good flow rates and high capacity.
- The industry standard for ion exchange chromatography.
- Compatible with single-pump configurations and chromatography systems such as AKTA design.
- Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
Storage and Stability
Analysis Note
Other Notes
Legal Information
related product
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Protocols
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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