G8634

Guanosine 5′-[γ-thio]triphosphate tetralithium salt

≥90% (contains < 10% GDP, HPLC), G-protein activator, powder

• Synonym(s): GTPγS tetralithium salt, GTP-γ-S-Li₄, Guanosine 5′-O-(3-thiotriphosphate) tetralithium salt
• Empirical Formula (Hill Notation): C₁₀H₁₂Li₄N₅O₁₃P₃S
• CAS Number: 94825-44-2
• Molecular Weight: 562.98
• NACRES: NA.77
• PubChem Substance ID: 24895344
• UNSPSC Code: 41106305
• EC Number: 305-606-1
• MDL number: MFCD00135151
• Beilstein/REAXYS Number: 8182249
• Assay: ≥90% (contains < 10% GDP, HPLC)
• Form: powder

Properties

• Product Name: Guanosine 5′-[γ-thio]triphosphate tetralithium salt, ≥90% (contains < 10% GDP, HPLC), powder
• assay: ≥90% (contains < 10% GDP, HPLC)
• form: powder
• color: white to off-white
• solubility: H₂O: ≥75 mg/mL (Solutions are very unstable; prepare immediately prior to use.)
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [Li+].[Li+].[Li+].[Li+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=S)[C@@H](O)[C@H]3O)C(=O)N1
• InChI: 1S/C10H16N5O13P3S.4Li/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(26-9)1-25-29(19,20)27-30(21,22)28-31(23,24)32;;;;/h2-3,5-6,9,16-17H,1H2,(H,19,20)(H,21,22)(H2,23,24,32)(H3,11,13,14,18);;;;/q;4*+1/p-4/t3-,5-,6-,9-;;;;/m1..../s1
• InChI key: AMQXJFWJOAWCPV-ZVQJTLEUSA-J

Description

Application

Guanosine 5′-[γ-thio]triphosphate tetralithium salt has been used:

• as a component of lysis buffer
• in oocyte and mammalian cell patch-clamp experiments to keep the G proteins in a constantly active state[1]
• to couple with G- protein α, to study its effect on kinase activity

Biochem/physiol Actions

GTP-γ-S is a non-hydrolyzable GTP analog, known for its role as a G-protein activator. It has recently been shown to protect proteins from proteolytic degradation, stimulate GLUT4 translocation in a tyrosine kinase-dependent manner, stimulate phospholipases and induce actin polymerization in vitro.

Non-hydrolyzable GTP analog, a G-protein activator.

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves