Sigma-Aldrich lists several fluorouracil products, but we do not offer Fluorouracil for injection. Our 5-Fluorouracil (5-FU) products cannot be given to human patients. (Note that although Product F8423 meets USP testing specifications, it is not a pharmaceutical grade or PharmaGrade product.)Having said that, a solution for injection into research animals could be prepared the same way as the Fluorouracil Injection, USP is. According to the drugs.com, "Fluorouracil Injection, USP, an antineoplastic anti-metabolite, is a colorless to yellow aqueous sterile, nonpyrogenic injectable solution for intravenous administration. Each mL contains: 50 mg fluorouracil; pH is adjusted to 8.6 to 9.4 with sodium hydroxide".
F6627
5-Fluorouracil
≥99% purity (HPLC), powder
Synonym(s):
2,4-Dihydroxy-5-fluoropyrimidine, 5-FU, 5-Fluoro-2,4(1H,3H)-pyrimidinedione
Sign Into View Organizational & Contract Pricing
Select a Size
1 G
₪326.00
5 G
₪1,211.00
10 G
₪1,626.00
All Photos(4)
Select a Size
Change View
1 G
₪326.00
5 G
₪1,211.00
10 G
₪1,626.00
About This Item
Empirical Formula (Hill Notation):
C4H3FN2O2
CAS Number:
Molecular Weight:
130.08
Beilstein:
127172
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Recommended Products
Product Name
5-Fluorouracil, ≥99% (HPLC), powder
Quality Level
Assay
≥99% (HPLC)
form
powder
technique(s)
titration: suitable
color
white
mp
282-286 °C (dec.) (lit.)
solubility
1 M NH4OH: soluble
DMSO/DMF: soluble
methanol: soluble
εmax
7.07 at 265 nm in 0.1 M HCl
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
FC1=CNC(=O)NC1=O
InChI
1S/C4H3FN2O2/c5-2-1-6-4(9)7-3(2)8/h1H,(H2,6,7,8,9)
InChI key
GHASVSINZRGABV-UHFFFAOYSA-N
Gene Information
human ... TYMS(7298)
Looking for similar products? Visit Product Comparison Guide
Application
Biochem/physiol Actions
A potent antitumor agent that affects pyrimidine synthesis by inhibiting thymidylate synthetase, thus depleting intracellular dTTP pools. It is metabolized to ribonucleotides and deoxyribonucleotides, which can be incorporated into RNA and DNA.[4][5] Treatment of cells with 5-FU leads to an accumulation of cells in S-phase and has been shown to induce p53 dependent apoptosis.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Oral - Carc. 2
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Handbook of Drug Nutrient Interactions
Nutrition and Health
(2004)
Curcumin promotes apoptosis, increases chemosensitivity, and inhibits nuclear factor kappaB in esophageal adenocarcinoma.
Hartojo W
Translational Oncology, 3, 99-99 (2010)
Coulson CJ
Molecular Mechanisms Of Drug Action (1994)
Overexpression of microRNA-125b sensitizes human hepatocellular carcinoma cells to 5-fluorouracil through inhibition of glycolysis by targeting hexokinase II.
Jiang JX
Molecular Medicine Reports, 10, 995-995 (2014)
CCL2/CCR2 chemokine signaling coordinates survival and motility of breast cancer cells through Smad3 protein- and p42/44 mitogen-activated protein kinase (MAPK)-dependent mechanisms.
Fang WB et al.
The Journal of Biological Chemistry, 287, 36593-36593 (2012)
Articles
We presents an article on Autophagy in Cancer Promotes Therapeutic Resistance
DNA damage and repair mechanism is vital for maintaining DNA integrity. Damage to cellular DNA is involved in mutagenesis, the development of cancer among others.
-
Do you have 5-FU (5-Fluorouracil) for injection? If not, how can I prepare it from the powder?
1 answer-
Helpful?
-
-
Is Product F6627, 5-Fluorouracil, sterile?
1 answer-
No. No sterility or endotoxin testing has been performed. Solutions of the product can be sterile filtered before use.
Helpful?
-
-
Products F6627 and 47576 are stated to have 99% purity. What are the differences between the two 5-fluorouracil products?
1 answer-
The purity of Product No. F6627 is determined by TLC (thin layer chromatography). The purity of deleted product 47576 was determined by HPLC (high performance liquid chromatography).
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
I see that Product F6627, 5-Fluorouracil, is soluble at a concentration of 50 mg/mL in 1M ammonium hydroxide. Do you have any information regarding the solubility in water?
1 answer-
Although product F6627 is not tested for solubility in water, there should be some limited solubility. Product 47576 has been tested at 10 mg/mL in water, although heat had to be applied. Upon cooling it might be possible to observe some precipitation. No testing in water is performed at Sigma on a lot to lot basis. The product is more soluble at higher alkaline pH.
Helpful?
-
-
Why does the 5-Fluorouracil have no effect on my cell line, even at micromolar concentrations that should be more than high enough?
1 answer-
Some cells, and in particular cancer cells, may be resistant to 5-fluorouracil. Please see this paper Huanwen, W., et al., Molecular Cancer, 8, 125 (2009) which addresses this observation, as well as references 16 and 30 in this publication.If the cells are treated with the closely related gemcitabine (Sigma-Aldrich Product G6423), and cell growth is not inhibited by that drug either, you will have shown that the "problem" is with the cell line, and not with the drug(s).
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
