The method of extraction and purification of this product are considered proprietary. None of the reagents used in the production of this material contain potassium. However, any residual remaining from the original tissue has not been determined.
F4503
Ferritin from equine spleen
Type I, saline solution
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|---|---|
25 mg | Please contact Customer Service for Availability | ₪271.00 |
100 mg | Please contact Customer Service for Availability | ₪550.00 |
500 mg | Please contact Customer Service for Availability | ₪1,620.00 |
1 g | Please contact Customer Service for Availability | ₪3,243.00 |
About This Item
Form:
saline solution
Mol wt:
H subunit ~21 kDa, L subunit ~19 kDa
sterility
sterile-filtered
Quality Segment
type
Type I
form
saline solution
mol wt
H subunit ~21 kDa, L subunit ~19 kDa
color
red to brown
cation traces
Cd: ≤1% (as % of ferritin)
storage temp.
2-8°C
General description
A shell of 24 protein subunits (apoferritin) and a core Fe 3+ ions with a peptide MW of 440 kDa. Review of the ferritin-like superfamily of iron-storage proteins, virtually ubiquitous in life on earth.
Application
Biochem/physiol Actions
A ubiquitous iron storage protein that plays a key role in iron metabolism. It serves as an intracellular iron reserve (particularly in spleen, liver, intestinal mucosa, and bone marrow) and functions in iron detoxification.
A ubiquitous iron storage protein that plays a key role in iron metabolism. It serves as an intracellular iron reserve (particularly in spleen, liver, intestinal mucosa, and bone marrow) and functions in iron detoxification. Studies have shown that ferritin iron incorporation is mediated by a ferroxidase activity associated with ferritin H subunits and a nucleation center associated with ferritin L subunits. Release of iron from ferritin has an essential role in iron-dependent lipid peroxidation and may contribute to free radical-induced cell damage in vivo. Therefore, by binding iron, ferritin may function as an antioxidant.
1 of 1
This Item | |||
|---|---|---|---|
| description Type I, saline solution | description 0.2 μm filtered | description Type V, 10 μg/mL in 0.15 M NaCl, 10 mM Tris, pH 8.0, containing 0.1% sodium azide | description Ferritin, Apo-, Equine Spleen, CAS 9013-31-4, is a native protein shell of ferritin molecule lacking iron. |
| mol wt L subunit ~19 kDa, H subunit ~21 kDa | mol wt minor subunit MH 22,200, native ~481.2 kDa (24 subunits, approx. 20 kDa each), major subunit ML 19,889 | mol wt - | mol wt - |
| form saline solution | form liquid | form liquid | form lyophilized solid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 300 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| sterility sterile-filtered | sterility 0.2 μm filtered | sterility sterile-filtered | sterility - |
| cation traces Cd: ≤1% (as % of ferritin) | cation traces - | cation traces - | cation traces Fe: <0.01% |
Storage Class
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
Hi, I would like to know whether the ferritin sample (F4503) contains any potassium, either in the saline solution or as a residual ion from the equine spleen extraction process. Thanks
1 answer-
Helpful?
-
-
Hi, I've just purchased a Ferritin from equine spleen (liquid, 100mg, cas No:9007-73-2, MDL No: MFCD00081593), however, I didn't find how many mL this solution is on the product label. Can you please provide any information regarding volume? Thanks.
1 answer-
The concentration, and thus the volume, of the Ferritin will vary for individual lots. The concentration can be found on the lot specific Certificate of Analysis, which can be retrieved by entering the lot number here:
https://www.sigmaaldrich.com/product/sigma/f4503#product-documentationThe volume can be determined using the following formula:
Units provided / Units per mL = mL providedThe concentration of the Ferritin is currently specified to be ≤125mg/ml
https://www.sigmaaldrich.com/specification-sheets/115/477/F4503-BULK________SIGMA____.pdfHelpful?
-
-
Does it run as a 44kDa specie or 21kDa+19kDa in size exclusion chromatography?
1 answer-
This product is not analyzed by chromatography. However, historical data indicates the material runs primarily at 20-22kD and 13-15kD in SDS electrophoresis studies. Additional bands appear at the 3000 - 5000 molecular weight range.
Helpful?
-
-
What is the concentration of salt(s) in the saline solution carrying the protein?
1 answer-
The saline solution is a 0.15 M sodium chloride solution. The concentration of 0.15 M can also be written as 8.8 mg NaCl per mL, or the textbook "0.9%" for normal saline.
Helpful?
-
-
Hi, I was wondering if I could get the volume of ferritin present in each of the package sizes? Thank you
1 answer-
The concentration of this product is lot specific and will impact the final volume received. Typical lots range from 50 - 75 mg/mL. Please see the link below to review the product specification sheet:
https://www.sigmaaldrich.com/specification-sheets/115/477/F4503-BULK________SIGMA____.pdfSee below for potential volume ranges for each package size:
F4503-25MG (0.5 - 0.33 mL)
F4503-100MG (2 - 1.33 mL)
F4503-500MG (10 - 6.66 mL)
F4503-1G (20 - 13.33 mL)Helpful?
-
-
Has Product F4503, Ferritin from equine spleen, been cell culture tested?
1 answer-
The solution is sterile-filtered but has not been tested in cell culture.
Helpful?
-
-
What is the concentration of ferritin in solutions of Product F4503, Ferritin from equine spleen?
1 answer-
The concentration of ferritin is lot specific and can be found on the Certificate of Analysis (CofA) for the specific lot.
Helpful?
-
-
Where is iron located in the ferritin molecule of Product F4503, Ferritin from equine spleen?
1 answer-
Iron is located in the core of the ferritin molecule consisting of 24 subunits. Up to 4500 iron ions can be contained in this core.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-