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Fibronectin Proteolytic Fragment from human plasma

Name: Fibronectin Proteolytic Fragment from human plasma
Brand: SIGMA

lyophilized powder, 70 kDa

Synonym(s):

Fibronectin

About This Item

UNSPSC Code:

12352202

eCl@ss:

42020141

NACRES:

NA.75

MDL number:

MFCD00131062

Form:

lyophilized powder

Assay:

>90% (SDS-PAGE)

Biological source:

human plasma

Mol wt:

70 kDa

This product is discontinued. Contact Technical Servicefor assistance.

Properties

biological source

human plasma

Quality Segment

200

assay

>90% (SDS-PAGE)

form

lyophilized powder

mol wt

70 kDa

packaging

pkg of 0.5 mg

technique(s)

cell culture | mammalian: suitable

impurities

HIV and HBsAg, source material tested negative, Small proteolytic fragments, may contain traces

solubility

water: soluble ≥0.500 mg/mL, clear to slightly hazy, colorless

UniProt accession no.

P02751

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... FN1(2335)

Description

General description

Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. FN1 is mapped to human chromosome 2q35. Proteolytic fragments of fibronectin has a cell binding domain.

Application

Fibronectin Proteolytic Fragment from human plasma has been used in pre adsorbed antibody blocking assay and EC-pericyte tube coassembly.

Biochem/physiol Actions

Fibronectin participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis.[1] Proteolytic fragments of fibronectin plays a vital role in mononuclear phagocyte function.

Preparation Note

This product is lyophilized from phosphate buffered saline with sucrose as a cryoprotectant. The source material has tested negative for antibody to HIV, HCN, and HBsAg. It is soluble in water at 0.5 mg/mL and yields a clear to slightly hazy solution.

Disclaimer

The product should be stored at -20°C.

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This Item	F0895	10838039001	ECM001
Sigma-Aldrich F0287 Fibronectin Proteolytic Fragment from human plasma	Sigma-Aldrich F0895 FIBRONECTIN FROM HUMAN PLASMA	Roche 10838039001 Fibronectin	Sigma-Aldrich ECM001 Fibronectin human
biological source human plasma	biological source human plasma	biological source human plasma	biological source human
assay >90% (SDS-PAGE)	assay -	assay 95% (SDS-PAGE)	assay ≥95% (SDS-PAGE)
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
mol wt 70 kDa	mol wt -	mol wt 440 kDa	mol wt 259.5 kDa (The protein migrates as a higher band on SDS-PAGE due to glycosylation.)
form lyophilized powder	form liquid	form lyophilized (clear, colorless solution after reconstitution)	form lyophilized powder
storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C