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E3031

Sigma-Aldrich

Monoclonal Anti-dEGF Receptor, Cytoplasmic Domain antibody produced in mouse

~1.5 mg/mL, clone E-257, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-Drosophila Epidermal Growth Factor Receptor, Anti-dEGFR

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About This Item

MDL number:
MFCD08705582
UNSPSC Code:
51111800

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

E-257, monoclonal

form

buffered aqueous solution

species reactivity

Drosophila

concentration

~1.5 mg/mL

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.25-0.5 μg/mL using recombinant cytoplasmic domain fragment of the Drosophila EGFR (amino acids 1200-1267)

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Drosophila melanogaster ... Egfr(37455)

General description

The signalling by dEGFR (also known as Torpedo or DER) is crucial for the development processes in Drosophila. The dEGFR signalling results in the activation of the canonical RAS/RAF/MEK/MAPK cascade. A variety of modulators such as Gro, TGF-α, Argos, Sprouty and Notch play an integrative role in EGFR signalling. The other pathways EGFR is involved in the development of the compound eye, larval, eye, wing and malphigian tubule development. Monoclonal Anti-dEGFR, Cytoplasmic Domain recognizes Drosophila Epidermal Growth Factor Receptor (EGFR). The antibody epitope resides within amino acids 1200-1267 of Drosophila EGFR.

Immunogen

recombinant cytoplasmic domain fragment of the Drosophila EGFR (amino acids 1200-1267).

Application

Anti-dEGF Receptor, Cytoplasmic Domain antibody may be used for immunoblotting at a working concentration of 0.25-0.5 μg/ml. The antibody is suitable for immunohistochemistry and ELISA.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Richard W Carthew
Current opinion in genetics & development, 17(4), 309-313 (2007-07-10)
The insect compound eye is one of the most precise and highly ordered patterns in the living world. It develops from an unpatterned simple epithelium by a series of cell fate decisions and complex morphogenetic movements. In the first days
Andrew Tomlinson et al.
Developmental biology, 454(2), 181-189 (2019-06-18)
Sevenless (Sev) is a Receptor Tyrosine Kinase (RTK) that is required for the specification of the Drosophila R7 photoreceptor. Other Drosophila photoreceptors are specified by the action of another RTK; the Drosophila EGF Receptor (DER). Why Sev is required specifically
P Hasson et al.
British journal of cancer, 94(6), 771-775 (2006-03-02)
In this minireview, we briefly revisit the Drosophila Notch and epidermal growth factor receptor pathways, and relate to the relationship between them. We then mainly focus on the involvement of Groucho (Gro)/TLE, a global developmental corepressor, in these pathways. In
Ben-Zion Shilo
Development (Cambridge, England), 132(18), 4017-4027 (2005-08-27)
The epidermal growth factor receptor (EGFR) signaling cascade represents one of the cardinal pathways that transmits information between cells during development in a broad range of multicellular organisms. Most of the elements that constitute the core EGFR signaling module, as

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