D0547

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): DME/F-12, 1:1 mixture
• UNSPSC Code: 12352207
• NACRES: NA.71
• MDL number: MFCD00217417

Properties

• Quality Level: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: NaHCO₃: no; sodium pyruvate: 0.11 g/L; HEPES: no; phenol red: yes; glucose: 3.15 g/L; L-glutamine: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

• to culture cerebral cortices from mice embryos
• as a component of the human corneal epithelium (HCE) media for culturing human limbal explants
• to culture human corneal epithelial cell lines[1]

Preparation Note

Formulated to contain 12.0 grams of powder per liter of medium.

Supplement with 1.2 g/L sodium bicarbonate

Other Notes

Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate