8D12 and 8A12 used in a sandwich ELISA giving a sensitive assay for biological samples. The antibody is directed against the guinea pig major basic protein found in guinea pig eosinophils. It can be paired in a sandwich ELISA with 8A12.
Maintain cultures between 1-2x100,000 cells/ml; 5% CO2; 37°C. Saturation density is reached at 1x1000,000 cells/ml.
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