Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.
Application
DNA transfection studies
NIH 3T3 Cell Line has been used to:
study calcium-mediated actin reset (CaAR) in response to physiological changes[1]
study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation[2]
determine the viral titer of the retroviral construct -ST40hTNFα[3]
Culture Medium
DMEM + 2mM Glutamine + 10% Calf Serum (CS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.
Other Notes
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DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
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