THM 7.3.5
Reactivity: Anti-NP (4-hydroxy-3 nitrophenacetyl), 87120201
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About This Item
UNSPSC Code:
12352200
biological source
Mouse + Human Transfectoma
ImmunoDon
Not applicable
Myeloma
J558L
Reactivity
Anti-NP (4-hydroxy-3 nitrophenacetyl)
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse J558L + Mouse VH gene + Human C(μ) gene
Cell Line Description
This human chimeric antibody is specifically directed against the hapten-4-hydroxy-3-nitrophenacetyl. V lambda of the endogenous light chain of plasmacytoma line complements VNP of transfected heavy chain to yield specific antibody. Gene marker: gpt.
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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