PLC/PRF/5

85061113, human liver, epithelial

• Synonym(s): Alexander cell line
• UNSPSC Code: 41106514

Properties

• product name: PLC/PRF/5, from human liver, 85061113
• biological source: human liver
• growth mode: Adherent
• karyotype: Not specified
• morphology: epithelial
• products: Hepatitis B Surface Antigen (HBSAg)
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Description

Alexander cell line which produces Hepatitis B antigen. The virus genome may be inducible in selective media. The cells should be handled under laboratory containment level 2. Expresses c-abl, c-fes, c-fms, c-myc, c-ha-ras and c-sis oncogenes. However there is currently no evidence that this cell line produces infectious Hepatitis B virus.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 13
D5S818: 12
D7S820: 9,11
THO1: 7,8
TPOX: 8
vWA: 15,16

Culture Medium

DMEM + 2mM Glutamine + 10% fetal bovine serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6; i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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