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TAT-1

00020911, Reactivity: Anti-α-Tubulin

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About This Item

UNSPSC Code:
41106514
Pricing and availability is not currently available.

biological source

Mouse x Mouse Hybridoma

clone

TAT-1

ImmunoDon

Mouse spleen

Myeloma

Sp2-0/Ag

Reactivity

Anti-α-Tubulin

technique(s)

cell culture | mammalian: suitable

isotype

IgG12b + IgG2a

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse x Mouse hybridoma

Cell Line Description

TAT-1 was developed by immunizing mice with extracted T. brucei brucei cytoskeleton and fusing the mouse spleen cells with the myeloma Sp2-0/Ag. This cell line has been cloned many times and the mixed subclass is now typical of the cloned line.

Immunogen

T. brucei brucei cytoskeleton (detergent extracted)

Application

Detection of α tubulin.

Culture Medium

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Subculture Routine

Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO2; 37°C

Other Notes

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