C6788

4-Chloro-1-naphthol

peroxidase substrate, tablet, suitable for use in immunoblotting procedures

• Synonym(s): 4-CN, 4-chloronaphthalen-1-ol
• Linear Formula: ClC₁₀H₆OH
• CAS Number: 604-44-4
• Molecular Weight: 178.61
• NACRES: NA.83
• PubChem Substance ID: 57654035
• UNSPSC Code: 12352204
• EC Number: 210-068-5
• MDL number: MFCD00003974
• Beilstein/REAXYS Number: 509750

Properties

• Product Name: 4-Chloro-1-naphthol, tablet
• form: tablet
• Quality Segment: 200
• mp: 118-121 °C (lit.)
• solubility: methanol: 10 mL, clear, colorless
• storage temp.: −20°C
• SMILES string: Oc1ccc(Cl)c2ccccc12
• InChI: 1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H
• InChI key: LVSPDZAGCBEQAV-UHFFFAOYSA-N

Description

Application

4-Chloro-1-naphthol has been used as a substrate in enzyme-linked immunospot (ELISpot) assay for detection of the spots.[1] It has also been used as a precipitating chromogenic substrate for visualizing the antigen−antibody-reactive bands following western blotting.

Biochem/physiol Actions

4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually.

Preparation Note

Contains 30 mg substrate per tablet.

Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 μl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves