The stability of this material following multiple freeze/thaw cycles indicates that a fine precipitate may be evident. However, the performance of the material was is effected. While it is recommended to use this product within 5 days of the initial thaw, it would be up to the end user to continue use following refreezing. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/241/461/c6295pis.pdf
C6295
Cell Freezing Medium-DMSO Serum free 1x
sterile-filtered, suitable for cell culture
Synonym(s):
cryopreservation medium
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50 ML
₪543.00
₪543.00
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50 ML
₪543.00
About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
₪543.00
Please contact Customer Service for Availability
Recommended Products
sterility
sterile-filtered
Quality Level
form
liquid
technique(s)
cell culture | mammalian: suitable
cryopreservation: suitable
impurities
endotoxin, tested
shipped in
dry ice
storage temp.
−20°C
General description
Cell freezing medium is a combination of cryoprotectant DMSO with fetal bovine serum. However serum free medium replaces serum with methylcellulose and poloxamer 188 and other additives like ascorbic acid, α-tocopherol and proline.[1]
Application
Other Notes
Complete ready-to-use medium containing Minimum Essential Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to the formulation of Waymouth[5].
related product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Overview of good cell banking practices for cell line cryopreservation purposes.
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Is it possible to thaw this product, prepare aliquots and store them at -20 degrees for further use? Thank you.
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