Unfortunately, a protocol for dilution is not available. However, the following information from the product manual may be helpful. Some types of primary human cells, especially Normal Human Epidermal Keratinocytes, respond sensitively to the calcium concentration in the culture medium. Calcium Chloride solution allows the adjustment of the optimal amount of calcium required by the cells. The Calcium Chloride (CaCl2) Solution is prepared as a solution from highly pure tissue culture tested Calcium Chloride and cell culture-grade water. It is a clear, colorless, and sterile solution. The concentration of CaCl2 is 0.5 M, and each vial contains 2 mL.
C-34006
Calcium Chloride Solution
CaCl 2-Solution (0.5 M), 2 ml
Synonym(s):
Calcium Chloride Liquid
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2 ML
₪209.00
About This Item
UNSPSC Code:
12352207
NACRES:
NA.81
form:
solution
Recommended Products
form
solution
packaging
pkg of 2 ml
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−20°C
General description
CaCl 2-Solution
Application
Some types of primary human cells, especially Normal Human Epidermal Keratinocytes (NHEK), respond sensitively to the calcium concentration in the culture medium. Calcium Chloride solution allows the adjustment of the optimal amount of calcium required by the cells. PromoCell Calcium Chloride (CaCl2) Solution is prepared as a solution from highly pure tissue culture tested Calcium Chloride and cell culture-grade water. It is a clear colorless and sterile solution.The concentration of CaCl2 is 0.5 M.
Quality
All lots of PromoCell Calcium Chloride Solution are subjected to comprehensive quality control tests. Each lot is routinely tested for the use with human primary cells, absence of cytotoxicity, and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria).
Storage and Stability
Store at -20°C in the dark immediately after arrival. If stored properly, the product is stable until the expiry date stated on the label.
Subculture Routine
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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I received this product for my master assay, and I saved it in -20 C. What should i do in order to use it and to change its concentration? Thank you so much for your time,
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