B0684

Anti-Bad antibody produced in rabbit

affinity isolated antibody, lyophilized powder

• Synonym(s): Anti-BBC2, Anti-BCL2L8
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD03791437
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 4

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: mouse, human
• technique(s): western blot: 1 μg/mL
• UniProt accession no.: Q92934
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... BAD(572) ; mouse ... Bad(12015)

Description

General description

Bad (Bcl-2-antagonist of cell death), initially identified by its interaction with Bcl-2 and Bcl-XL, is a distant Bcl-2 family member. Bad bears only the most universally conserved amino acids within BH1 and BH2 domains, and lacks the typical hydrophobic C-terminal signal anchor.

Immunogen

synthetic peptide corresponding to amino acids 1-21 of human Bad conjugated to KLH.

Application

Anti-Bad antibody produced in rabbit is suitable for western blotting at a concentration of 1μg/mL

Biochem/physiol Actions

The protein encoded by this gene forms heterodimers with BCL-xL and BCL-2 and replaces Bax, which in turn inverts the death repressor activity of Bcl-xL and positively regulates cell apoptosis.Bad is phosphorylated on two serine residues embedded in canonical 14-3-3 binding sites in response to IL-3, a survival factor. Phosphorylated Bad does not bind Bcl-XL and is sequestered in the cytosol bound to 14-3-3, a specific phosphoserine-binding protein. The growth factors that promote cell survival activate the threonine kinase Akt, which phosphorylates Bad causing suppression of apoptosis.

Physical form

lyophilized from a 0.2 μm filtered solution of phosphate buffered saline (PBS) and 5% trehalose

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)