A6284

Human IgG−Agarose

suspension

• Synonym(s): IgG Agarose
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00147242
• Conjugate: agarose conjugate
• Species reactivity: -
• Application: —
• Citations: 14

Properties

• conjugate: agarose conjugate
• Quality Segment: 200
• form: suspension
• extent of labeling: ≥5 mg per mL
• matrix: Cross-linked 4% beaded agarose
• matrix activation: cyanogen bromide
• matrix spacer: 1 atom
• storage temp.: 2-8°C

Description

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.

Application

Tandem affnitiy purification pulldown was performed in yeast cultures using agarose-linked human IgG. Supernatants were mixed with 30μl of agarose beads overnight.

IgG-Agarose is an immuno-adsorbent that can be used to affinity purify antibodies, remove species-specific cross-reacting antibodies or remove contaminating antibodies from an antiserum preparation. Typically, cross-reacting antibodies may be removed from an antiserum preparation using an equal volume of IgG-Agarose (resin volume). However, the resin to antiserum ratio will vary with individual applications.

Physical form

Suspension in 0.5 M NaCl containing preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable