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A3151

Avidin–Peroxidase

lyophilized powder

Synonym(s):

Avidin-POD

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Size/SKUAvailabilityPrice
1 mg
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₪663.00
2 mg
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₪1,072.00
5 mg
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₪2,299.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00130587
Form:
lyophilized powder
Biological source:
avidin from egg white, enzyme from horseradish

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Quality Segment

200

biological source

avidin from egg white, enzyme from horseradish

conjugate

peroxidase conjugate

form

lyophilized powder

composition

Protein, ≥70% E1%/280

extent of labeling

0.7-2.0 mol peroxidase per mol avidin

technique(s)

direct ELISA: 1:50,000

solubility

H2O: soluble 1 mg/mL, PBS: soluble

storage temp.

−20°C

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues[1]. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin-Peroxidase has been used for detecting anti-5-bromodeoxyuridine (anti-BrdU) binding[2] and OVA-IgG1[3].
To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees.

Physical form

Lyophilized powder containing citrate buffer.

Preparation Note

Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
A7419A2901189725
Avidin–Peroxidase lyophilized powder

Sigma-Aldrich

A3151

Avidin–Peroxidase

Avidin–Peroxidase buffered aqueous solution

Sigma-Aldrich

A7419

Avidin–Peroxidase

Avidin–FITC from egg white lyophilized powder

Sigma-Aldrich

A2901

Avidin–FITC from egg white

Avidin, Egg White

Sigma-Aldrich

189725

Avidin, Egg White

biological source

avidin from egg white, enzyme from horseradish

biological source

avidin from egg white, enzyme from horseradish

biological source

-

biological source

chicken

technique(s)

direct ELISA: 1:50,000

technique(s)

indirect ELISA: 1:100,000, western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well)

technique(s)

immunohistochemistry: suitable

technique(s)

-

form

lyophilized powder

form

buffered aqueous solution

form

lyophilized powder

form

lyophilized solid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

H2O: soluble 1 mg/mL, PBS: soluble

solubility

-

solubility

-

solubility

dilute buffer: 5 mg/mL, distilled water: 5 mg/mL

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

FITC conjugate

conjugate

-

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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