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957D

DAB Substrate Kit

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About This Item

NACRES:
NA.32
UNSPSC Code:
12161503
Technique(s):
immunohistochemistry: suitable
Storage temp.:
2-8°C
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description

For In Vitro Diagnostic Use in Select Regions

Quality Segment

500

form

aqueous solution

packaging

pkg of 1000 tests (957D-60), pkg of 150 tests (957D-50), pkg of 2000 tests (957D-30), pkg of 500 tests (957D-20), pkg of 5000 tests (957D-40)

manufacturer/tradename

Cell Marque®

technique(s)

immunohistochemistry: suitable

storage temp.

2-8°C

General description

DAB Substrate Kit is intended for use in immunohistochemistry (IHC) staining protocols. This reagent is designed to detect target antigens in formalin-fixed, paraffin-embedded tissue sections when used in conjunction with primary antibodies and appropriate secondary antibody conjugates in the IHC staining process.When in the presence of HRP polymer, DAB produces a brown precipitate that can be readily visualized using light microscopy. Kits contain: DAB chromogen and DAB buffer substrate.

Physical form

Ready to use reagent

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Analysis Note

United States - IVD
Canada - IVD
European Union - IVD
Japan - RUO

Other Notes

For Technical Service please contact: 800-665-7284 or email: [email protected]

Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H341,H350

Hazard Classifications

Carc. 1B - Muta. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

1–3 of 3 Questions  

  1. how to prep DAB?

    1 answer

    1. it is one drop of chromogen to 1ml of substrate buffer.

      Helpful?

  2. What is the volume for "1 drop" of DAB chromogen?

    1 answer

    1. The volume used to prepare 1ml of DAB would be 0.006mls, rounded to 0.01mls based on calculations for the number of tests in the kit 957D-20.

      Helpful?

  3. Is the absence of color normal when 1 drop of chromagen and 1 ml of substrate are used, given the usual light purple color?

    1 answer

    1. Ensure thorough mixing of the chromagen before combining. The color outcome is influenced by the chromogen's age. A lack of color typically suggests very recent or fresh DAB, which has not undergone oxidation.

      Helpful?

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