421M-1

p21^WAF1 (DCS-60.2) Mouse Monoclonal Antibody

• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: DCS-60.2, monoclonal
• Application: IHC (p)
• Citations: 8

Properties

• biological source: mouse
• Quality Segment: 100, 500
• conjugate: unconjugated
• antibody form: diluted ascites fluid
• antibody product type: primary antibodies
• clone: DCS-60.2, monoclonal
• description: For In Vitro Diagnostic Use in Select Regions (See Chart)
• form: buffered aqueous solution
• species reactivity: human
• packaging: vial of 0.1 mL concentrate (421M-14), vial of 0.5 mL concentrate (421M-15), bottle of 1.0 mL predilute (421M-17), vial of 1.0 mL concentrate (421M-16), bottle of 7.0 mL predilute (421M-18)
• manufacturer/tradename: Cell Marque^®
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200
• isotype: IgG2a
• control: colon
• shipped in: wet ice
• storage temp.: 2-8°C
• visualization: nuclear
• Gene Information: human ... CDKN1A(1026)

Description

General description

p21 is a nuclear 21 kD protein, a product of the WAF1/CIP1 gene. It is a cyclin-dependent kinase inhibitor 1A (p21, Cip1), also known as CDKN1A, which in humans is encoded by the CDKN1A gene located on chromosome (6p21.2). It is a constituent of a large complex of nuclear proteins, including cyclins, cyclin dependent kinases, and PCNA. Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 inhibits the activity of each member of the cyclin/Cdk family. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Analysis Note

IVD

IVD

IVD

RUO

Other Notes

For Technical Service please contact: 800-665-7284 or email: [email protected]

p21WAF1 Positive Control Slides, Product No. 421S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany