116R-1

CD16 (SP175) Rabbit Monoclonal Antibody

• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: SP175, monoclonal
• Application: IHC (p)
• Citations: 5

Properties

• biological source: rabbit
• Quality Segment: 100, 500
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: SP175, monoclonal
• description: For In Vitro Diagnostic Use in Select Regions (See Chart)
• form: buffered aqueous solution
• species reactivity: human
• packaging: vial of 0.1 mL concentrate (116R-14), vial of 0.5 mL concentrate (116R-15), bottle of 1.0 mL predilute (116R-17), vial of 1.0 mL concentrate (116R-16), bottle of 7.0 mL predilute (116R-18)
• manufacturer/tradename: Cell Marque^®
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
• isotype: IgG
• control: tonsil
• shipped in: wet ice
• storage temp.: 2-8°C
• visualization: cytoplasmic, membranous
• Gene Information: human ... FCGR3A(2214)

Description

General description

CD16 is a biomarker associated with monocytes and natural killer (NK) cells of the lymphoid lineage.[1] Anti-CD16 immunohistochemistry is useful in differentially diagnosing hepatosplenic gamma delta T-cell lymphoma and gamma delta T-cell large granular lymphocyte leukemia from other peripheral T-cell lymphomas, such as mucosal and cutaneous gamma delta T-cell lymphoma.[2][3][4] It is reported that 58% of hepatosplenic gamma delta T-cell lymphomas express CD16, and 86% of gamma delta T-cell large granular lymphocyte leukemias are immunoreactive with anti-CD16.[2][3] Mucosal and cutaneous gamma delta T-cell lymphomas usually do not express CD16 antigen.[4] A significant decrease can be seen in the number of granulocytes expressing CD16 in chronic myelomonocytic leukemia compared to chronic myelogenous leukemia and control bone marrow biopsy, probably related to dysgranulopoiesis.[5] Bone marrow biopsy immunohistochemistry can be helpful in CMML by identifying both the monocyte expansion and the dysgranulopoiesis with anti-CD16.[5]

CD16 is a receptor on monocytes and natural killer (NK) cells, which facilitates antibody-dependent cellular cytotoxicity.[1] CD16 is a useful marker in NK neoplasms and in certain T-cell neoplasms such as T-cell large granular lymphocytic leukemia.[1]

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Analysis Note

IVD

IVD

IVD

RUO

Other Notes

CD16 Positive Control Slides, Product No. 116S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

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Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany