02744

Atto 655-Streptavidin

BioReagent, suitable for fluorescence

• Synonym(s): Streptavidin-Atto 655
• NACRES: NA.32
• UNSPSC Code: 12352116

Properties

• product line: BioReagent
• Quality Segment: 100
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• solubility: DMF: soluble, DMSO: soluble, acetonitrile: soluble
• fluorescence: λ_ex 655 nm; λ_em 680 nm in 0.1 M phosphate pH 7.0
• suitability: suitable for fluorescence, suitable for gel electrophoresis
• storage temp.: −20°C

Description

Application

Atto 655 is a fluorescent label with high molecular absorption (125.000) and quantum yield (0.30), as well as sufficient stoke′s shift (excitation maximum 663 nm, emission maximum 684 nm). There is very little cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Atto 655 has been coupled to streptavidin to enable various types of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays.

Analysis Note

free of unconjugated dye

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)