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02744

Atto 655-Streptavidin

BioReagent, suitable for fluorescence

Synonym(s):

Streptavidin-Atto 655

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Pack SizeSKUAvailabilityPrice
1 mg
Please contact Customer Service for Availability
₪2,091.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352116

₪2,091.00

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product line

BioReagent

Quality Level

100

form

powder

manufacturer/tradename

ATTO-TEC GmbH

solubility

DMF: soluble, DMSO: soluble, acetonitrile: soluble

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence, suitable for gel electrophoresis

storage temp.

−20°C

Application

Atto 655 is a fluorescent label with high molecular absorption (125.000) and quantum yield (0.30), as well as sufficient stoke′s shift (excitation maximum 663 nm, emission maximum 684 nm). There is very little cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Atto 655 has been coupled to streptavidin to enable various types of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays.

Analysis Note

free of unconjugated dye

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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This Item
0926006966RSTREP-RO
Sigma-Aldrich

Sigma-Aldrich

02744

Atto 655-Streptavidin

Sigma-Aldrich

Sigma-Aldrich

09260

Atto 425-Streptavidin

Sigma-Aldrich

Sigma-Aldrich

06966

Atto 655-Biotin

Streptavidin, recombinant liquid, ≥17 units/mg protein, pH range 5.0-9.0

Roche

RSTREP-RO

Streptavidin, recombinant

suitability

suitable for fluorescence, suitable for gel electrophoresis

suitability

-

suitability

suitable for fluorescence

suitability

suitable for immunological tests

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 437 nm; λem 477 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

fluorescence

-

form

powder

form

powder

form

powder

form

liquid

solubility

DMF: soluble, acetonitrile: soluble, DMSO: soluble

solubility

H2O: soluble

solubility

DMF: soluble, DMSO: soluble, acetonitrile: soluble

solubility

water: 10 mg/L (mA405=100)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-10 to -25°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

-

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM6542
BCCL7578
BCCK3312
BCCJ3542
BCCF1140

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Articles

Atto Dyes for Superior Fluorescent Imaging

Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.

Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving.
Bretschneider, S.; Eggeling, Chr.; Hell, S. W.
Physical Review Letters, 98(21) (2007)
Simple buffers for 3D STORM microscopy.
van de Linde, S.; Kasper, R.; Heilemann, M.; Sauer, M.
Applied Physics B: Lasers and Optics, 93(4), 725-731 (2008)
Triple-Color Super-Resolution Imaging of Live Cells. Resolving Submicroscopic Receptor Organization in the Plasma Membrane.
Wilmes, S.; Staufenbiel, M.; Lisse, D.; Richter, Ch. P.; Beutel, O.; Busch, K. B. et al.
Angewandte Chemie (International Edition in English), 124(20), 4952-4955 (2012)
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Global Trade Item Number

SKUGTIN
W276413-1KG-K04061835565955
W276413-SAMPLE04061833904251
W276405-100G-K04061835513468
W276405-SAMPLE-K04061837516405
W276413-SAMPLE-K04061834355656
W276405-25KG-K04061834404811
800399010004022536358376
PHR1124-1G04061834413493
M3128-500G04061834046400
G100120-500G04061832011301
G100120-50G04061833624630
G100120-10G04061838551528
70082-250G04061832860589
1448990-200MG04061838735973
W276405-1KG-K04061835565948

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