43291

m-FC Agar, Vegitone

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Membrane Fecal Coliform Agar, Vegitone, Vegitone m-FC Agar, m-Fecal Coliform Agar, Vegitone
• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15.0 g/L ; anilne blue, 0.1 g/L ; lactose, 12.5 g/L ; proteose peptone (vegetable), 5.0 g/L ; sodium chloride, 5.0 g/L ; synthetic detergent, 1.5 g/L ; tryptose (vegetable), 10.0 g/L ; yeast extract, 3.0 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.4±0.2 (25 °C)
• application(s): agriculture; bioburden testing; environmental; food and beverages; water monitoring; microbiology
• suitability: selective and differential for Escherichia coli; selective and differential for Salmonella spp.; selective and differential for Shigella spp.; selective and differential for coliforms

Description

Application

M-FC Agar is used for the detection and enumeration of faecal coliforms using membrane filter technique at higher temperature. In this modified medium the animal derived peptones are replaced by plant peptones.

Preparation Note

Dissolve 52.1 g in 1 litre distilled water and heat to boiling to dissolve completely. Add 10 ml of a 1 % solution of rosolic acid (Cat. No. 83910) in 0.2 N NaOH. Continue heating for 1 minute with frequent agitation. Do not autoclave! Cool to 45-50°C. Dispense 4 ml amounts into Petri dishes (50-60mm) and allow to solidify.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable